Effectiveness of Bdellovibrio Against the Immune System of White Shrimp (Litopenaues vannamei) Infeksion with Vibrio harveyi Bacteria

Authors

  • I Wayan Rainbow Mataram University
  • Fariq Azhar Program Studi Budidaya Perairan, Fakultas Pertanian, Universitas Mataram
  • Andre Rachmat Scabra Program Studi Budidaya Perairan, Fakultas Pertanian, Universitas Mataram

DOI:

https://doi.org/10.29303/mediaakuakultur.v5i1.6081

Abstract

White shrimp (Litopenaeus vannamei) is an aquatic biota that is included in the leading commodity of aquaculture. The Vibrio harveyi bacteria can cause up to 80% death in white shrimp. The predatory Bdellovibrio is highly regarded as an alternative source of antibiotics and has been reported to have the potential to control shrimp pathogens such as Vibrio chalerae and Vibrio parahaemolyticus. The aim of this study was to analyze the effect of giving Bdellovibrio on the immune system of vaname shrimp infected with Vibrio harveyi bacteria. This research is experimental using a completely randomized design (CRD), which consists of 5 treatments and 3 replications. This study was experimental using a completely randomized design (CRD), which consisted of 5 treatments and 3 replications. Treatments in the study included P1 (+)(Not given Bdellovibrio and tested with Vibrio harveyi), P2 (-) (Not given Bdellovibrio and tested with NaCL), P3 (Given 30 ppm Bdellovibrio and tested with Vibrio harveyi), P4 (Given Bdellovibrio 50 ppm and tested with Vibrio harveyi), and P5 (Given Bdellovibrio 70 ppm and tested with Vibrio harveyi). In this study, the best treatment value was obtained, namely P5 treatment, the THC value was 16.94 x 106 cells/ml, the DHC value in hyaline cells was 59.33%, the granular cells were 24%, the semi-granular cells were 16.67%, the AF value 64.82%, TBC value 5.98 x 108 CFU/ml, TVC value 1.43 x 107 CFU/ml, FCR value 1.23, specific length growth rate 19.4%/day, specific weight growth rate 9 .96%/day, the absolute length value is 8.73 cm, the absolute weight is 61 grams and the SR value is 77.78%. From this research, it was concluded that administration of Bdellovibrio in the rearing media at different doses was able to improve the immune system and survival of White shrimp tested with Vibrio harveyi bacteria

References

Adiyodi, K. G., & Adiyodi, R. G. (1970). Endocrine Control of Reproduction in Decapod Crustacea. Biological reviews of the Cambridge Philosophical Society, 45(2), 121–164

Aguilera-Rivera, D., Escalante-Herrera, K., Gaxiola, G., Prieto-Davó, A., Rodríguez-Fuentes, G., Guerra-Castro, E., Hernández-López, J., Chávez-Sánchez, M. C., & Rodríguez-Canul, R. (2019). Immune Response of the Pacific White Shrimp, Litopenaeus vannamei, Previously Reared in Biofloc and After an Infection Assay With Vibrio harveyi. Journal of the World Aquaculture Society, 50(1), 119–136. https://doi.org/10.1111/jwas.12543

Agustama, Y., Lestari, T.A., Verdian, A.H., Witoko, P., & Marlina, E. 2021. Penambahan Probiotik EM4 dan Bacillus sp Pada Pakan Buatan Terhadap Pertumbuhan dan Kelangsungan Hidup Post Larva Udang Vaname. Jurnal Perikanan Terapan. 2(1): 39-44.

Apriliani, M., Sarjito, & Haditomo, A. H. C. (2016). Keanekaragaman Agensia Penyebab Penyakit Vibriosis pada Udang Vaname (Litopenaeus vannamei) dan Sensitivitasnya Terhadap Antibiotik. Journal of Aquaculture Management and Technology, 5(1), 98–107.

Arianto, R. M., Fitri, A. D. P., & Jayanto, B. B. (2018). Pengaruh Aklimatisasi Kadar Garam Terhadap Nilai Kematian dan Respon Pergerakan Ikan Wader (Rasbora argyrotaenia) Untuk Umpan Hidup Ikan Cakalang. Journal of Fisheries Resources Utilization Management and Technology, 7(2), 43–51.

Azhar F. 2018. Aplikasi Bioflok yang Dikombinasikan dengan Probiotik untuk Pencegahan Infeksi Vibrio parahaemolyticus pada Pemeliharaan Udang Vaname (Litopenaeus vannamei). Journal of Aquaculture Science3: 128–137.

Azhari A., Muchlisin Z.A., Dewiyanti I. (2017). Pengaruh Padat Penebaran Terhadap Kelangsungan Hidup dan Pertumbuhan Benih Ikan Seurukan (Osteochilus vittanus). Jurnal Ilmiah Mahasiswa Kelautan dan Perikanan Unsyiah2: 12–19.

Broberg, C. A., Calder, T. J., Orth, K. (2011). Review Vibrio parahaemolyticus cell biology and pathogenicity determinants. Microbes and Infection, 13, 992-10

Cao, H., Wang, H., Yu, J., An, J., & Chen, J. (2019). Encapsulated bdellovibrio powder as a potential bio-disinfectant against whiteleg shrimp-pathogenic vibrios. Microorganisms, 7(8). https://doi.org/10.3390/microorganisms7080244

Central Statistics Agency, 2017. Shrimp exports by main destination countries 2000-2015. https://www. bps.go.id/LinkTabelSt%20atis/view /id/1015.

Citria, I. (2018). Pengaruh Penggunaan Probiotik yang Difermentasikan Dengan Sumber Karbon yang Berbeda Terhadap Pertumbuhan Udang Vaname (Litopenaeus vannamei). Universitas Mataram.

Dugassa, H., & Gaetan, D. G. (2018). Biology of White Leg Shrimp, Penaeus vannamei: Review. World Journal of Fish and Marine Sciences, 10(2), 5–17.

Duncan, M. C., Forbes, J. C., Nguyen, Y., Shull, L. M., Gillette, R. K., Lazinski, D. W., Ali, A., Shanks, R. M. Q., Kadouri, D. E., & Camilli, A. (2018). Vibrio cholerae motility exerts drag force to impede attack by the bacterial predator Bdellovibrio bacteriovorus. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-07245-3

Dwidar, M., Monnappa, A. K., & Mitchell, R. J. (2012). The dual probiotic and antibiotic nature of Bdellovibrio bacteriovorus. In BMB Reports (Vol. 45, Issue 2, pp. 71–78). https://doi.org/10.5483/BMBRep.2012.45.2.71

Ekasari, J., Azhar, M.H., Surawidjaja, E.H., Nuryati, S., De Schryver, P., & Bossier, P. (2014). Immune Response and Disease Resistance of Shrimp Fed Biofloc Grown On Different Carbon Sources. Fish & shellfish immunology. 41(2): 332-339

Ekawati, A. W., Nursyam, H., Widjayanto, E., & Marsoedi, M. (2012). Diatomae Chaetoceros ceratosporum dalam Formula Pakan Meningkatkan Respon Imun Seluler Udang Windu (Penaeus monodon Fab.). The Journal of Experimental Life Sciences, 2(1), 20–28. https://doi.org/10.21776/ub.jels.2012.002.01.04

Ernawati, E., & Rochmady, R. (2017). Effect of Fertilization and Density on The Survival Rate and Growth of Post-larva of Shrimp Vaname (Litopenaues vannamei). Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil, 1(1), 1.

Fao, Matthew Briggs, Funge-Smith, S., Subasinghe, R., & Phillips, M. (2004). Introductions and Movement of Penaeus vannamei and Penaeus stylirostris in Asia and the Pacific. In RAP publication. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3119427&tool=pmcentrez&rendertype=abstract

Fendjalang, S. N. M., Budiardi, T., Supriyono, E., & Effendi, I. (2016). Produksi Udang Vaname (Litopenaeus vannamei) Pada Karamba Jaring Apung dengan Padat Tebar Berbeda Di Selat Kepulauan Seribu. Jurnal Ilmu Dan Teknologi Kelautan Tropis, 8(1), 201–214.

Fuandila, N. N., Widanarni, W., & Yuhana, M. (2019). Growth Performance and Immune Response of Prebiotic Honey Fed Pacific White Shrimp Litopenaeus vannamei to Vibrio parahaemolyticus Infection. Journal of Applied Aquaculture. https://doi.org/https://doi.org/10.1080/104554438.2019.1615593

Harahap, F. R., Kardhinata, H., & Mutia, H. (2017). Inventory of Shrimp in The Waters Kampung Nipah Kecamatan Perbaungan Kabupaten Serdang Bedagai North Sumatra. Jurnal Biologi, 3(2), 92–102.

Huisman EA. (1987). Principles of fish production. Department of Fish Culture and Fisheries, Wageningen Agriculture University. Wageningen. Netherland. 170p.

Ismawati, I., Destryana, R. A., & Huzaimah, N. (2019). Imunitas Udang Vanname (Litopenaeus vannamei) yang Diberi Pakan Tambahan Daun Kasembukan (Paederia foetida Linn.). Jurnal Kelautan, 12(2), 201–206. https://doi.org/10.21107/jk.v12i2.5998

Jannah, M., Junaidi, M., Setyowati, D. N., & Azhar, F. (2018). Pengaruh Pemberian Lactobacillus sp. dengan Dosis yang Berbeda terhadap Sistem Imun Udang Vaname (Litopenaeus vannamei) yang diinfeksi Bakteri Vibrio parahaemolyticus. Jurnal Kelautan: Indonesian Journal of Marine Science and Technology, 11(2), 140. https://doi.org/10.21107/jk.v11i2.3980

Johansson, M. W., Keyser, P., Sritunyalucksana, K., & Söderhäll, K. (2000). Crustacean Haemocytes and Haematopoiesis. Aquaculture, 191(1–3), 45–52. https://doi.org/10.1016/S0044-8486(00)00418-X

Junaidi, M., Azhar, F., Setyono, B. D. H., & Waspodo, S. (2020). Pengaruh Pemberian Ekstrak Daun Mangrove Rizhophora apiculata terhadap Performa Pertumbuhan Udang Vaname. Buletin Veteriner Udayana, 4(21), 198. https://doi.org/10.24843/bulvet.2020.v12.i02.p15

Kaligis, E. Y. (2010). Peningkatan Sintasan dan Kinerja Pertumbuhan Udang Vaname (Litopenaeus vannamei, Boone) Di Media Bersalinitas Rendah. Intitut Pertanian Bogor.

Kaligis, E. Y. (2010). Peningkatan Sintasan dan Kinerja Pertumbuhan Udang Vaname (Litopenaeus vannamei, Boone) Di Media Bersalinitas Rendah. Intitut Pertanian Bogor.

Kharisma, A., & Manan, A. (2012). Kelimpahan Bakteri Vibrio sp. Pada Air Pembesaran Udang Vannamei (Litopenaeus vannamei) Sebagai Deteksi Dini Serangan Penyakit Vibriosis. Jurnal Ilmiah Perikanan dan Kelautan, 4(3).

Kordi M. G. H. K. (2011). Budidaya 22 Komuditas Laut Untuk Konsomsi Lokal Dan Ekspor. Yogyakarta: Lily Publisher.

Koval, S. F., & Hynes, S. H. (1991). Effect of Paracrystalline Protein Surface Layers on Predation by Bdellovibrio bacteriovorus. In JOURNAL OF BACTERIOLOGY (Vol. 173, Issue 7). https://journals.asm.org/journal/jb

Kristina, Y. (2014). Analisis Faktor-Faktor Yang Mempengaruhi Produksi dan Pendapatan Budidaya Tambak Udang Vaname Di Kecamatan Pasekan Kabupaten Indramayu.

Kusmarwati, A., Yennie, Y. & Indriati, N., (2017). Resistensi Antibiotik Pada Vibrio parahaemolyticus Dari Udang Vaname Asal Pantai Utara Jawa Untuk Pasar Ekspor. JPB Kelautan dan Perikanan, 12(02), 91-106.

Manoppo, H. et al., 2011. Peningkatan Respon Imun Non-Spesifik, Resistensi, dan Pertumbuhan Udang vaname (Litopenaeus vannamei) Melalui Pemberian Pakan Nukleotida. Jurnal Akuakultur Indonesia, (10), 1-7.

Manoppo, H., & Kolopita, M. E. F. (2014). Respon imun krustase. Budidaya Perairan, 2(2), 22–26.

Muthaiyan, R., Krishnan, S., Periyasamy, S., Chetri, Z., & Nandakumar, R. (2020). Immune Response of Shrimp Peneaus monodon Against Vibrio parahaemolyticus. April. www.worldnewsnaturalsciences.com

Nishibuchi, M., & Kaper, J.B. (1995). Thermostable direct hemolysin gene of V. parahaemolyticus: a virulence gene acquired by a marine bacterium. Infect Immun, 63(6), 2093–2099

Nuhman. 2008. Pengaruh Prosentase Pemberian Pakan Terhadap Kelangsungan Hidup Dan Laju Pertumbuhan Udang Vannamei (Litopenaeus vannamei). Jurnal Berkala Ilmiah Perikanan. 3 (1), 1-16.

Ode, I., (2013). Kajian Sistem Imuitas Untuk Pengendalian Penyakit Pada Ikan Dan Udang. Jurnal Ilmiah Agribisnis dan Perikanan, 6(2), 41- 43.

Panjaitan, A. S. (2012). Pemeliharaan Larva Udang Vaname (Litopenaeus vannamei, Boone 1931) Dengan Pemberian Jenis Fitoplankton Yang Berbeda

Putri, F. M., Sarjito, S., & Suminto, S. (2013). Pengaruh Penambahan Spirulina sp. Dalam Pakan Buatan terhadap Jumlah Total Hemosit dan Aktivitas Fagositosis Udang Vaname (Litopenaeus vannamei). Journal of Aquaculture Management and Technology, 2(1), 102–112.

Rahim N., Wulan S., Zaenuddin E.N. (2020). Isolasi Metabolit Sekunder dari Daun Kawista. Jurnal Ilmu Farmasi3: 159–161.

Ridlo, A. & Pramesti, R., (2009). Aplikasi Ekstrak Rumput Laut Sebagai Agen Imunostimulan Sistem Pertahanan Non Spesifik Pada Udang (Litopenaeus vannamei). Ilmu Kelautan, 14(3), 133-137.

Rodríguez, J., & Le Moullac, G. (2000). State of the art of Immunological Tools and Health Control of Penaeid Shrimp. Aquaculture, 191(1–3), 109–119. https://doi.org/10.1016/S0044-8486(00)00421-X

Rosyida A., Azhar F., Setyowati D.N. (2022). Pengaruh Penambahan Ekstrak Kunyit Putih (Curcuma zedoaria) Terhadap Sistem Imun Udang Vaname (Litopenaeus vannamei) yang Diuji Tantang dengan Bakteri Vibrio harveyi. Jurnal Perikanan dan Kelautan 27: 136–144.

Starr, M. P., & Baigent, N. L. (1966). Parasitic Interaction of Bdellovibrio bacteriovorus with Other Bacteria. In JOURNAL OF BACTERIOLOGY (Vol. 91, Issue 5). https://journals.asm.org/journal/jb

Sudheesh, P. & Xu, H., (2001). Pathogenicity of Vibrio parahaemolyticus in Tiger Prawn Penaeus monodon Fabricius: Possible Role of Extracellular Proteases. Journal Aquaculture 196, p. 37–46.

Suleman, Andayani S., Yuniarti A. (2019). Potensi Ekstrak Kasar Ulva lactuta dalam Meningkatkan Total Haemocyte Count (THC) dan Aktivitas Fagositosis pada Udang Vaname (Litopenaeus vannamei). Jurnal Ilmu Perikanan10: 1–7.

Taslihan, A., Callinan, R., Torribio, J.-A., Sumiarto, B., & Nitimulyo, K. H. (2015). Cluster Model For Extensive Giant Tiger Shrimp (Penaeus monodon Fab) To Prevent Transmission Of White Spot Syndrome Virus. Indonesian Aquaculture Journal14(1)

Verschuere, L., G. Robaut., P. Sorgeloos and W. Verstraete. (2000). Probiotic Bacteri As (Starr & Baigent, 1966)Biological Control Agents in Aquaculture. A Reviews. Microbiology and Molecular Biology. 64(4):655-671. https://doi.org/10.1128/MMBR.64.4.655-671.2000

Vet, Chin J. (2003). Clinical experiment technical practice for fishery drugs. Ministry of Agriculture of China 37, 11–14.

Vieira, R. H. S. F., Costa, R. A., Menezes, F. G. R., & Maggioni, R. (2011). Kanagawa-Negative, tdh- and trh-Positive Vibrio parahaemolyticus Isolated from Fresh Oysters Marketed in Fortaleza, Brazil. Curr Microbiology, 63,126-130. doi: 10.1007/s00284-011- 9945-x

Wachid, B. A. A., Setyowati, D. N., & Azhar, F. (2022). Effectiveness of Meniran Leaf Extract (Phyllanthus niruri L.) as Immunostimulant in Vannamei Shrimp (Litopenaeus vannamei) Against Vibriosis Disease. Journal of Aquaculture and Fish Health, 11(2), 182–192. https://doi.org/10.20473/jafh.v11i2.28672

Widagdo, P. (2011). Aplikasi Probiotik, Prebiotik, dan Sinbiotik Melalui Pakan Pada Udang Vaname (Litopenaeus vannamei) yang Diinfeksi Bakteri Vibrio Harveyi. Fakultas Perikanan dan Ilmu Kelautan. Institut Pertanian Bogor

Widanarni, Sukenda, M. Setiawati. (2008). Bakteri Probiotik dalam Budidaya Udang: Seleksi, Mekanisme Aksi, Karakterisasi, dan Aplikasinya Sebagai Agen Biokontrol. Jurnal Ilmu Pertanian Indonesia, 13(2), 80-89. ISSN: 0853- 4217

Xu, W.J., & Pan, L.Q. (2013). Enhancement of Immune Response and Antioxidant Status of Litopenaeus vannamei Juvenile In Biofloc-Based Culture Tanks Manipulating High C/N Ratio of Feed Input. Aquaculture. 412:117-124

Downloads

Published

2025-03-27

How to Cite

I Wayan Rainbow, Azhar, F., & Scabra, A. R. (2025). Effectiveness of Bdellovibrio Against the Immune System of White Shrimp (Litopenaues vannamei) Infeksion with Vibrio harveyi Bacteria. Indonesian Journal of Aquaculture Medium, 5(1), 55–79. https://doi.org/10.29303/mediaakuakultur.v5i1.6081

Most read articles by the same author(s)

1 2 > >>