THE EFFECT OF TYPES OF SOAKING MEDIUM AND PRETREATMENT COMBINATION ON CHEMICAL, PHYSICAL, AND ORGANOLEPTIC QUALITIES OF RED BEAN FLOUR

Authors

  • Nur Adha Febriandini
  • Eko Basuki
  • Dody Handito

Keywords:

soaking, steam-blanching, red bean flour

Abstract

This research aims to determine the effect of soaking medium type and pre-treatment combinations on the physical, chemical, and organoleptic quality of red bean flour. This research was conducted using a Randomized Block Design (RBD) with single factor: type of soaking medium and the combination of pre-treatments, as follows: J1M1 (soaking in distilled water), J1M2 (soaking in distilled water and steam-blanching), J2M1 (soaking in 0.25% baking soda), J2M2 (soaking in 0.25% baking soda and steam-blanching), J3M1 (soaking in 1.25% baking powder), and J3M2 (soaking in 1.25% baking powder and steam-blanching). The parameters tested were moisture content, protein content, yield, fineness, color (L* value and hue angle), and organoleptic properties (appearance, aroma, taste, and overall quality). The test results were analyzed using analysis of variance (ANOVA) with a significance level of 5%. If there was a significant effect, it was further tested using the Tukey's Honestly Significant Difference (HSD) at a 5% level. The best treatment was obtained by soaking in 1.25% baking powder and steam-blanching, resulting in red bean flour with high sensory quality and acceptance levels for appearance, aroma, taste, and overall quality, with a flour fineness of 99.99% passing thru a 60-mesh sieve and a moisture content of 5.40% meeting the quality requirements of BPOM Regulation No. 34 of 2019; a protein content of 24.61%; a yield of 57.21%, high brightness (L*=92.03), and a yellowish color (°hue=69.55).

References

Abdel-Aleem, W. M., Sanaa M. Abdel-Hameed, and Souzan S. Latif. 2019. Effect of Soaking and Cooking on Nutritional and Quality Properties of Faba Bean. J. of Food and Dairy Sci. 10 (10): 389-395. https://doi.org/10.21608/jfds.2019.62862.

Association of Official Analytical Collaboration (AOAC). 2001. AOAC Official Method 2001.11. Protein (Crude) in Animal Feed, Forage (Plant Tissue), Grain, and Oilseeds: Block Digestion Method Using Copper Catalyst and Steam Distillation into Boric Acid. First Action 2001. Oxford University Press.

Ávila, Bianca P., Magda S. dos Santos, Angélica M. Nicoletti, Gabriela D. Alves, Moacir C. Elias, Jander Monks, and Márcia A. Gularte. 2015. Impact of Different Salts in Soaking Water on the Cooking Time, Texture and Physical Parameters of Cowpeas. Plant Foods Hum Nutr 70:463-469. https://doi.org/10.1007/s11130-015-0504-7.

Awuchi, C. G., Igwe, V. S., & Echeta, C. K. (2019). The Functional Properties of Foods and Flours. International Journal of Advanced Academic Research, 5(11), 139-160. https://api.semanticscholar.org/CorpusID:233305894

Badan Pusat Statistik. 2021. Produksi Tanaman Sayuran Menurut Provinsi dan Jenis Tanaman 2020. Badan Pusat Statistik.

Badan Standardisasi Nasional. 1992. Cara Uji Makanan dan Minuman. SNI 01-2891-1992. Badan Standardisasi Indonesia.

Badan Standardisasi Nasional. (1992). Cara Uji Makanan dan Minuman SNI 01-2891-1992. Badan Standardisasi Indonesia. https://akses-sni.bsn.go.id/viewsni/baca/957 Badan Pengawas Obat dan Makanan. 2019. Peraturan Badan Pengawas Obat dan Makanan Nomor 34 Tahun 2019 Tentang Kategori Pangan. Badan Pengawas Obat dan Makanan.

Badan Standardisasi Nasional. 2011. Petunjuk Pengujian Organoleptik dan atau Sensori. SNI 01-2346-2006_Rev. 2011. Badan Standardisasi Nasional.

Brown, Amy. 2011. Understanding Food: Principles and Preparation. N.p.: Cengage Learning. https://books.google.co.id/books/about/Understanding_Food_Principles_and_Prepar.html?id=G0ltCgAAQBAJ&redir_esc=y.

CHNSpec. 2015. Precise Color Reader Intruction Manual CS-10. N.p.: CHNSpec. https://images-na.ssl-images-amazon.com/images/I/A1gbzioXA3L.pdf.

Chong, Wah-Kang, Sook-Yun Mah, Azhar M. Easa, and Thuan-Chew Tan. 2019. Thermal Inactivation of Lipoxygenase in Soya Bean Using Superheated Steam to Produce Low Beany Flavour Soya Milk. J Food Sci Technol 56 (9): 4371-4379. https://doi.org/10.1007/s13197-019-03905-4.

Devi, Reena, Charul Chaudhary, Veena Jain, AK Saxena, and Sholpa Chawla. 2018. Effect of soaking on anti-nutritional factors in the sun-dried seeds of hybrid pigeon pea to enhance their nutrients bioavailability. Journal of Pharmacognosy and Phytochemistry 7 (2): 675-680.

Ertaş, Nilgün. 2021. Improving the cake quality by using red kidney bean applied different traditional processing methods. J Food Process Preserv 45 (e15527): 1-10. https://doi.org/10.1111/jfpp.15527.

Fauzi, Andi A., Muhsin Muhsin, dan Andi Sukainah. 2016. Pengaruh Variasi Larutan Perendaman Sukun terhadap Karakteristik Fisiko Kimia Tepung Sukun. Jurnal Pendidikan Teknologi Pertanian 2:79-86. http://dx.doi.org/10.26858/jptp.v2i0.5188.

Febrianto, Noor A., Khalimatus Sa'diyah, and Tejasari. 2016. Red Kidney Bean Powder Substituted Milk in Cinnamon Herbal Coffee: Consumer Perception, Sensory Properties and Nutrition Content. Pelita Perkebunan 32 (2): 109-119. https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v32i2.223.

Fennema, Owen R., Srinivasan Damodaran, and Kirk L. Parkin. 2017. Fennema's Food Chemistry, Fifth Edition. N.p.: CRC Press LLC. Boca Raton.

Godrich, Joseph, Peter Rose, Molly Muleya, and Joanne Gould. 2023. The effect of popping, soaking, boiling and roasting processes on antinutritional factors in chickpeas and red kidney beans. Int J Food Sci Technol 58:279-289. https://doi.org/10.1111/ijfs.16190.

Hoxha, Ibrahim, Gafur Xhabiri, and Ramadan Deliu. 2020. The Impact of Flour from White Bean (Phaseolus vulgaris) on Rheological, Qualitative and Nutritional Properties of the Bread. Open Access Library Journal 7 (e6059). https://doi.org/10.4236/oalib.1106059.

Hutchings, John B. 1999. Food Color and Appearance. Second ed. N.p.: Springer. Gaithersburg.

Kambabazi, M. R., M. W. Okoth, S. Ngala, L. Njue, and H. Vasanthakaalam. 2021. Evaluation of Nutrient Content In Red Kidney Beans, Amaranth Leaves, Sweet Potato Roots and Carrots Cultivated in Rwanda. Afr. J. Food Agric. Nutr. Dev. 21 (4): 17801-17814. https://doi.org/10.18697/ajfand.99.21095.

Kartiningsih, Kurnia M. Solichah, dan Faurina R. Fauzia. 2023. Nilai Gizi dan Daya Terima Cookies Wikara (Wijen Kacang Merah) untuk Mencegah Anemia Defisiensi Besi. Journal Health and Nutritions 9 (2): 80-92. http://dx.doi.org/10.52365/jhn.v9i2.790.

Kementerian Kesehatan. 2018. Komposisi Gizi Kacang Merah, kering (Kidney bean, kering). Database Pangan Kelompok Kacang-kacangan.

Kementerian Pertanian. 2023. Statistik Konsumsi Pangan Tahun 2023. N.p.: Kementerian Pertanian.

Kenar, James A., Frederick C. Felker, Mukti Singh, Jeffery A. Byars, Mark A. Berhow, Michael J. Browman, and Jill K. Winkler-Moser. 2020. Comparison of composition and physical properties of soluble and insoluble navy bean flour components after jet-cooking, soaking, and cooking. LWT - Food Science and Technology 130 (109765). https://doi.org/10.1016/j.lwt.2020.109765.

King, Jessie, Sze Y. Leong, Marbie Alpos, Courtney Johnson, Stephanie Mcleod, Mei Peng, Kevin Sutton, and Indrawati Oey. 2024. Role of food processing and incorporating legumes in food products to increase protein intake and enhance satiety. Trends in Food Science & Technology 147 (104466): 1-19. https://doi.org/10.1016/j.tifs.2024.104466.

Leahu, Ana, and Alice I. Rosu. 2014. Effect of soaking on the cooking quality and color parameters of common beans (Phaseolus Vulgaris L.). Food and Environment Safety 13 (3): 244-251. https://fens.usv.ro/index.php/FENS/article/view/107/105.

Locali-Pereira, Adilson R., Mirian T. Kubo, Caroline G. Fuzetti, and Vânia R. Nicoletti. 2022. Functional Properties of Physically Pretreated Kidney Bean and Mung Bean Flours and Their Performance in Microencapsulation of a Carotenoid-Rich Oil. Front. Sustain. Food Syst 6 (845566): 1-11. https://doi.org/10.3389/fsufs.2022.845566.

Lotfy, Shereen N., Rasha Saad, Khaled F. El-Massrey, and Hoda H. Fadel. 2021. Effects of pH on headspace volatiles and properties of Maillard reaction products derived from enzymatically hydrolyzed quinoa protein-xylose model system. LWT - Food Science and Technology 145 (111328): 1-9. https://doi.org/10.1016/j.lwt.2021.111328.

Lu, Jing, Galia Zamaratskaia, Maud Langton, Hanna S. Rohnisch, and Saeid Karkehabadi. 2024. Minimizing anti-nutritional factors in wet protein extraction from Swedish faba beans through the application of response surface methodology. Food Chemistry 460:140700. https://doi.org/10.1016/j.foodchem.2024.140700.

Lu, John Y., and Mohamed S. Al Jasser. 1986. Characteristics and Acceptability of a Flour Prepared from Sodium Bicarbonate Soaked and Steamed Soybeans. Journal of Food Processing and Preservation 10:177-187. https://doi.org/10.1111/j.1745-4549.1986.tb00017.x.

Majzoobi, Masha, Yasaman Pesaran, Gholamreza Mesbahi, and Asgar Farahnaky. 2016. Evaluation of the Effects of Hydrothermal Treatment on Rice Flour and Its Related Starch. International Journal of Food Properties 19 (9): 2135-2145. https://doi.org/10.1080/10942912.2015.1110165.

Mananga, Marlyne-Josephine, Eyili N. Karrington, Kotue T. Charles, Kouandjoua N. Didier, and Fokou Elie. 2022. Effect of Different Processing Methods on the Nutritional Value of Red and White Bean Cultivars (Phaseolus vulgaris L.). Journal of Food and Nutrition Sciences 10 (1): 27-35. https://doi.org/10.11648/j.jfns.20221001.15.

Mizobutsi, Gisele P., Fernando L. Finger, Rosiline A. Ribeiro, Rolf Puschmann, Ludmila Lafetá de Melo Neves, and Wagner Ferreira da Mota. 2010. Effect of pH and temperature on peroxidase and polyphenoloxidase activities of litchi pericarp. Sci. Agric. (Piracicaba, Braz.) 67 (2): 213-217. https://doi.org/10.1590/S0103-90162010000200013.

Mkanda, Alice V., Amanda Minnaar, and Henriette L. de Kock. n.d. Relating consumer preferences to sensory and physicochemical properties of dry beans (Phaseolus vulgaris). J. Sci. Food Agric. 87:2868-2879. https://doi.org/10.1002/jsfa.3046.

Muhammad, Dimas R., Diinah S. Kamiilah, Ardiba R. Sefrienda, Asri Nursiwi, and Anastriyani Yulviatun. 2025. Formulation of vegan chocolate with soybean and kidney bean: physical and sensory properties. IOP Conf. Ser.: Earth Environ. Sci. 1460 (012034). https://doi.org/10.1088/1755-1315/1460/1/012034.

Palupi, Hapsari T., Teti Estiasih, Yunianta Subroto, and Aji Sutrisno. 2024. The effects of alkaline extraction on the characteristics of lima bean (Phaseolus lunatus) starch. Brazilian Journal of Food Technology 27 (e2022018). https://doi.org/10.1590/1981-6723.01822.

Pangastuti, Hesti A., Dian R. Affandi, dan Dwi Ishartani. 2013. Karakterisasi Sifat Fisik dan Kimia Tepung Kacang Merah (Phaseolus Vulgaris L.) Dengan Beberapa Perlakuan Pendahuluan. Jurnal Teknosains Pangan 2 (1): 20-29. https://jurnal.uns.ac.id/teknosains-pangan/article/view/4204/3630.

Patil, Nikhil D., Aarti Bains, Kandi Sridhar, Summya Rashid, Sawinder Kaur, Nemat Ali, Prince Chawla, and Minaxi Sharma. 2024. Effect of Sustainable Pretreatments on the Nutritional and Functionality of Chickpea Protein: Implication for Innovative Food Product Development. Journal of Food Biochemistry 2024 (5173736): 1-29. https://doi.org/10.1155/2024/5173736.

Poste, Linda M., Deborah A. Mackie, Gail Butler, and Elizabeth Larmond. 1991. Laboratory Methods for Sensory Analysis of Food. N.p.: Canada Communication Group, Pub. Centre. Ottawa. https://publications.gc.ca/site/eng/9.699476/publication.html.

Prayudani, Ayu P., Budyawan Saputra, Made Astawan, Tutik Wresdiyati, and Ratnaningsih E. Sardjono. 2023. Effect of Pre-Milling Method on Physicochemical and Functional Properties of Velvet Bean (Mucuna pruriens L.) Flour. Food Science and Technology 11 (2): 111-124. https://doi.org/10.13189/fst.2023.110202.

Purnomo, Eko H., Achmad N. Ginanjar, Feri Kusnandar, dan Cynthia Andriani. 2015. Karakterisasi Sifat Fisikokimia Tepung Kacang Hitam dan Aplikasinya pada Brownies Panggang. Jurnal Mutu Pangan 2 (1): 26-33. https://journal.ipb.ac.id/index.php/jmpi/article/view/27867/17824.

Ratnawati, L., D. Desnilasari, D. N. Surahman, and R. Kumalasari. 2019. Evaluation of Physicochemical, Functional and Pasting Properties of Soybean, Mung Bean and Red Kidney Bean Flour as Ingredient in Biscuit. IOP Conf. Series: Earth and Environmental Science 251 (012026). https://dx.doi.org/10.1088/1755-1315/251/1/012026.

Samben, Resti K., and Dylla H. Puspaningrum. 2020. Kandungan Protein, Serat, dan Daya Terima Kacang Gude pada Perbedaan Perlakuan Suhu dan Waktu. Sintesa Prosiding, 135-144. https://doi.org/10.36002/snts.v0i0.1245.

Samuel, Reno, Intan N. Azni, dan Giyatmi. 2019. Pengaruh Substitusi Tepung Kacang Merah Terhadap Mutu Produk Brownies Kukus. J. Teknol. Pangan Kes. 1 (2): 113-121. https://jurnal.usahid.ac.id/teknologi_pangan/article/view/203/199.

Shashego, Zeritu. 2019. Effects of Soaking Time and Temperature on the Nutritional Content and Sensory Quality of Soybean Flour and Milk. Global Journal of Science Frontier Research 19 (D3): 25-28. https://journalofscience.org/index.php/GJSFR/article/view/2530.

Sparvoli, Francesca, Monica Laureati, Roberto Pilu, Ella Pagliarini, Ivan Toschi, Gianluca Giuberti, Paola Fortunati, Maria G. Daminati, Eleonora Cominelli, and Roberto Bollini. 2016. Exploitation of Common Bean Flours with Low Antinutrient Content for Making Nutritionally Enhanced Biscuits. Front. Plant Sci. 7 (928). https://doi.org/10.3389/fpls.2016.00928.

Stephany, Michael, Philipp Eckert, Stephanie Bader-Mittermaier, Ute Schweiggert-Weisz, and Reinhold Carle. 2016. Lipoxygenase Inactivation Kinetics and Quality-Related Enzyme Activities of Narrow-Leafed Lupin Seeds and Flakes. LWT - Food Science and Technology 68:36-43. http://dx.doi.org/10.1016/j.lwt.2015.11.052.

Sudarmadji, Slamet, Bambang Haryono, dan Suhardi. 2010. Analisa untuk Bahan Makanan dan Minuman. N.p.: Liberty. Yogyakarta.

Thakur, S., M. G. Scanlon, R. Tayler, A. Milani, and J. Paliwal. 2019. Pulse Flour Characteristics from a Wheat Flour Miller’s Perspective: A Comprehensive Review. Comprehensive Reviews in Food Science and Food Safety 18 (3): 775-797. https://doi.org/10.1111/1541-4337.12413.

Tiofanny, Valencia, and Wiwit Amrinola. 2025. Effect of Fermentation Modification Using Tempeh Starter on the Physical, Functional, and Pasting Properties of Sorghum Flour (Sorghum Bicolor L.). IOP Conference Series. Earth and Environmental Science 1485 (1): 1-12. https://dx.doi.org/10.1088/1755-1315/1485/1/012004.

Troszyńska, A., R. Amarowicz, G. Lamparski, A. Wołejszo, and N. Baryłko-Pikielna. 2005. Investigation of Astringency of Extracts Obtained from Selected Tannins-Rich Legume Seeds. Food Quality and Preference 1 17 (2006): 31-35. https://doi.org/10.1016/j.foodqual.2005.04.006.

Wodajo, Derese, and Shimelis A. Emire. 2022. Haricot Beans (Phaseolus Vulgaris L.) Flour: Effect of Varieties and Processing Methods to Favor the Utilization of Underconsumed Common Beans. International Journal of Food Properties 25 (1): 1186-1202. https://doi.org/10.1080/10942912.2022.2074029.

Ziena, H. M. 2007. Effect of Soaking Medium on Organoleptic Properties, Chemical Composition and Some Antinutritional Factors of Parched Chickpea (Cicer Arietinum L.). J. Agric. Sci. Mansoura Univ 32 (2): 1217-1227. https://doi.org/10.21608/jfds.2007.198701.

Published

2025-12-31