The Effect of Recombinant Growth Hormone Administration on Bonylip Barb (Osteochilus vittatus) Fry at Diferent Stocking Densities.
DOI:
https://doi.org/10.29303/mediaakuakultur.v5i2.7339Keywords:
ikan nilem, recombinant growth hormone, padat tebarAbstract
The nilem fish (Osteochilus vittatus) has high economic value, but its growth rate is relatively slow. One potential solution to accelerate its cultivation period is the use of recombinant growth hormone (rGH). This study aimed to evaluate the effect of oral administration of rGH derived from Epinephelus lanceolatus (rElGH) on the growth rate of nilem fish juveniles at different stocking densities. A completely randomized design with three replications was used in this study. Juvenile fish with an average length of 4.43±0.03 cm and weight of 0.96±0.03 g were reared in aquaria measuring 100 × 50 × 50 cm with a water height of 20 cm for 30 days. The fish were fed commercial feed containing 31–33% protein, with rElGH-supplemented feed given to satiation twice daily (at 08:00 and 16:00) during the first seven days, followed by regular feed without rElGH. The best production performance was obtained in the R30 treatment group (30 fish per 100 L with rElGH), with an absolute weight gain (AWG) of 3.4 g, a survival rate of 98.89%, and a specific growth rate (SGR) of 4.8% per day, indicating that nilem fish given rElGH grew faster.References
Acosta, J., Carpio, Y., Besada, V., Morales, R., Sanchez, A., Curbelo, Y., Ayala, J., & Estrada,
M., P. (2008). Recombinant truncated tilapia growth hormone enhances growth
and innate immunity in tilapia fry (Oreochromis sp.). General and Comparative
Endocrinology. (157): 49-56.
Acosta, J., Morales, R., Morales, A., Alonso, M., & Estrada, M. P. (2007). Pichia pastoris
expressing recombinant tilapia growth hormone accelerates the growth of tilapia.
Biotechnology Letters, 29(11), 1671–1676. https://doi.org/10.1007/s10529-007-
9424-z
Alimuddin, Handoyo, N. B. P., & Utomo. (2014). Efektivitas pemberian hormon
pertumbuhan rekombinan ikan kerapu kertang (Epinephelus lanceolatus, Bloch
1790) melalui perendaman dan oral terhadap pertumbuhan elver ikan sidat
(Anguilla bicolor bicolor). Jurnal Iktiologi Indonesia, 14(3), 179–188
Alimuddin, Lesmana, I., Sudrajat, A. O., Carman, O., & Faizal, I. (2010). Production and
bioactivity potential of three recombinant growth hormones of farmed fish.
Indonesian Aquaculture Journal, 5(1), 11–16.
Apriliana, R., Basuki, F., & Agung, R. (2017). Pengaruh pemberian recombinant growth
hormone (rGH) dengan dosis berbeda pada pakan buatan terhadap pertumbuhan
dan kelulushidupan benih ikan tawes (Puntius sp.). Jurnal Sains Akuakultur Tropis,
2(1), 49–57.
Atmojo, A., Basuki, F., & Nugroho, R. A. (2017). Pengaruh pemberian rekombinan hormon
pertumbuhan (rGH) melalui metode perendaman dengan lama waktu yang berbeda
terhadap pertumbuhan dan kelulushidupan larva bawal air tawar (Colossoma
macropomum Cuv). Journal of Aquaculture Management and Technology, 6(3), 1–8.
Boyd, C. E. (2015). Water quality: An introduction. Springer.
Caputo, M., Pigni, S., Agosti, E., Daffara, T., Ferrero, A., Filigheddu, N., & Prodam, F. (2021).
Regulation of GH and GH signaling by nutrients. Cells, 10(6), 1–38.
https://doi.org/10.3390/cells10061440
Conde-Sieira, M., Chivite, M., Míguez, J. M., & Soengas, J. L. (2018). Stress effects on the
mechanisms regulating appetite in teleost fish. Frontiers in Endocrinology, 9, 1–7.
https://doi.org/10.3389/fendo.2018.00631
Debnath, S. (2010). A review on the physiology of insulin-like growth factor-I (IGF-I)
peptide in bony fishes and its phylogenetic correlation in 30 different taxa of 14
families of teleosts. Advances in Environmental Biology, 5, 31–51.
Effendie, M. I. (1979). Biologi perikanan. Bogor, Indonesia: Yayasan Dewi Sri.
Effendi, M. I. (2002). Biologi perikanan. Bogor, Indonesia: Yayasan Dewi Sri.
Goddard, S. (1996). Feed management in intensive aquaculture. New York, NY: Chapman
and Hall. https://doi.org/10.1007/978-1-4613-1173-7
Handayani, F. (2021). Pembenihan dan pembesaran ikan nilem (Osteochilus hasselti) di
Cabang Dinas Kelautan dan Perikanan Wilayah Selatan (CDKPWS), Tasikmalaya,
Jawa Barat [Undergraduate thesis, Institut Pertanian Bogor].
Handoyo, B. (2012). Metode pemberian dan respons benih ikan sidat terhadap hormon
pertumbuhan rekombinan ikan kerapu kertang [Master's thesis, Institut Pertanian
Bogor].
Hardiantho, D., Alimuddin, Prasetiyo, A. E., Yanti, D. H., & Sumantadinata, K. (2012).
Performa benih ikan nila diberi pakan mengandung hormon pertumbuhan
rekombinan ikan mas dengan dosis berbeda. Jurnal Akuakultur Indonesia, 11(1), 17–
21.
Hu, Y. B., Mai, K., Ai, Q., Zheng, S., & Cheng, K. (2008). Growth and body composition of
juvenile white shrimp (Litopenaeus vannamei) fed different ratios of dietary protein
to energy. Aquaculture Nutrition, 14, 499–506. https://doi.org/10.1111/j.1365-
2095.2007.00555.x
Irmawati, Alimuddin, Zairin, M., Suprayudi, M. A., & Wahyudi, A. T. (2012). Peningkatan
laju pertumbuhan benih ikan gurame (Osphronemus gouramy Lac.) yang direndam
dalam media yang mengandung hormon pertumbuhan ikan mas. Jurnal Iktiologi
Indonesia (in press).
Iskandar, & Elrifadah. (2015). Pertumbuhan dan efisiensi pakan ikan nila yang diberi
pakan buatan berbasis kiambang. Jurnal Ziraa’ah, 40(1), 18–24.
Latar, D. I. (2013). Efektivitas pemberian hormon pertumbuhan rekombinan melalui pakan
dengan bahan penyalut berbeda dan pelleting pada ikan nila [Master's thesis, Institut
Pertanian Bogor]
28
Long, L., Zhang, H., Ni, Q., Liu, H., Wu, F., & Wang, X. (2019). Effects of stocking density on
growth, stress, and immune responses of juvenile Chinese sturgeon (Acipenser
sinensis) in a recirculating aquaculture system. Comparative Biochemistry and
Physiology Part C: Toxicology & Pharmacology, 219, 25–34.
https://doi.org/10.1016/j.cbpc.2019.02.002
Moriyama, S., Hiroshi, Y., Seiji, S., Toshio, A., Tetsuya, H., & Hiroshi, K. (1993). Oral
administration of recombinant salmon growth hormone to rainbow trout
(Oncorhynchus mykiss). Aquaculture, 112(1), 99–106.
https://doi.org/10.1016/0044-8486(93)90060-C
Moriyama, S., & Kawauchi, H. (1990). Growth stimulation of juvenile salmonids by
immersion in recombinant salmon growth hormone. Nippon Suisan Gakkaishi,
56(1), 31–33. https://doi.org/10.2331/suisan.56.31
Mumpuni, F. S., Muarif, Yulianti, N., & Hilmy, A. M. (2022). The growth, feed efficiency, and
survival rate of bonylip barb (Osteochilus hasselti) in biofloc media C/N ratio 10 with
different stocking densities. Journal of Aquaculture and Fish Health, 11(2), 227–237.
https://doi.org/10.20473/jafh.v11i2.30814
Nurkarina, R. (2013). Kualitas media budidaya dan produksi ikan nilem (Osteochilus
hasselti) yang dipelihara pada sistem IMTA (Integrated Multi Trophic Aquaculture)
dengan kepadatan berbeda [Undergraduate thesis, Institut Pertanian Bogor].
Pratama, A. E., Lumbessy, S. Y., & Azhar, F. (2021). Pengaruh pemberian pakan komersial
dengan campuran recombinant growth hormone (rGH) pada budidaya ikan kakap
putih (Lates calcarifer). Jurnal Kelautan, 14(2), 164–174.
https://doi.org/10.21107/jk.v14i2.10334
Promdonkoy, B., Warit, S., & Panyim, S. (2004). Production of a biologically active growth
hormone from giant catfish (Pangasianodon gigas) in Escherichia coli. Biotechnology
Letters, 26(8), 649–653. https://doi.org/10.1023/B:BILE.0000019565.74563.49
Putra, A. N. (2015). Basal metabolism in fish. Jurnal Perikanan dan Kelautan, 5(2), 57–65.
https://doi.org/10.33512/jpk.v5i2.1065
Rafaey, M. M., Li, D., Tian, X., Zhang, Z., Zhang, X., Li, L., & Tang, R. (2018). High stocking
density alters growth performance, blood biochemistry, intestinal histology, and
muscle quality of channel catfish (Ictalurus punctatus). Aquaculture, 492, 73–80.
https://doi.org/10.1016/j.aquaculture.2018.04.021
Rakhfid, A., Baya, N., Bakri, M., & Fendi, F. (2017). Pertumbuhan dan kelangsungan hidup
udang vaname (Litopenaeus vannamei) pada padat tebar berbeda. Jurnal
Akuakultur, 1(2), 1–5.
Ritonga, L. B. R. (2020). Pengaruh padat tebar yang berbeda terhadap laju pertumbuhan
ikan wader cakul (Puntius binotatus). Jurnal Chanos Chanos, 18(1), 1–5.
Said, D. S., Mayasari, N., & Crismadha, T. (2020). Potential of endemic and native fish from
Maninjau Lake, West Sumatra, Indonesia as a nutritional source. Ecology,
Environment, and Conservation Journal, 26(2), 20–25.
Saputra, A., Budiardi, T., Samsudin, R., & Rahmadya, N. D. (2018). Growth performance
and survival of snakehead (Channa striata) juvenile with different stocking density
reared in recirculation system. Jurnal Akuakultur Indonesia, 17(2), 104–112.
https://doi.org/10.19027/jai.17.2.104-112
Sinaga, V. O., & Mukti, R. C. (2022). The growth of tilapia (Oreochromis niloticus) with the
addition of probiotics to feed in Sakatiga Village, Indralaya District, Ogan Ilir
Regency, South Sumatera. Journal of Aquaculture and Fish Health, 11(1), 90–96.
https://doi.org/10.20473/jafh.v11i1.27610
29
Sutiana, Erlangga, & Zulfikar. (2017). Pengaruh dosis hormon rGH dan tiroksin dalam
pakan terhadap pertumbuhan dan kelangsungan hidup benih ikan koi (Cyprinus
carpio, L). Aquatic Sciences Journal, 4(2), 76–82.
Wicaksono, P. (2005). Pengaruh padat tebar terhadap pertumbuhan dan kelangsungan
hidup ikan nilem (Osteochilus hasselti C.V.) yang dipelihara dalam keramba jaring
apung di Waduk Cirata dengan pakan perifiton [Undergraduate thesis, Institut
Pertanian Bogor].
Wong, A. O. L., Hong, Z., Yonghua, J., Wendy, K., & Ko, W. (2006). Feedback regulation of
growth hormone and secretion in fish and the emerging concept of intrapituitary
feedback loop (review). Comparative Biochemistry and Physiology Part A: Molecular
& Integrative Physiology, 144, 284–300.
https://doi.org/10.1016/j.cbpa.2006.03.016
Yuniarti, I., Susilowati, T., & Faozi, O. (2022). Pengaruh pemberian recombinant growth
hormone (rGH) melalui pakan dengan interval waktu yang berbeda terhadap
pertumbuhan dan sintasan benih ikan tawes (Puntius javanicus). Jurnal Riset
Akuakultur, 17(1), 35–45. https://doi.org/10.15578/jra.17.1.2022.35-45
Zheng, J. L., Zhang, H. T., Gao, L., Chen, X., Zhu, Q. L., & Han, T. (2024). Combined effects of
crowding stress and low salinity on GH/IGF axis, antioxidant response, and HPI axis
in largemouth bass (Micropterus salmoides) larvae. Aquaculture, 578, 740036.
https://doi.org/10.1016/j.aquaculture.2024.740036