Comparative Analysis of Chlorella vulgaris Cell Densities in Different Culture Systems
DOI:
https://doi.org/10.29303/mediaakuakultur.v5i1.6920Keywords:
Chlorella vulgaris, Culture Scale, Biomass productivity, water quality, aquaculture feedAbstract
Chlorella vulgaris is a cosmopolitan microalga with high nutritional value, widely utilized in aquaculture, pharmaceuticals, and biofuel production. This study evaluated its growth performance across three culture scales (laboratory bottle, carboy, and intermediate concrete tank) at BPBAP Situbondo from February to June 2024. The research aimed to analyze the influence of scale on biomass productivity and identify critical environmental factors. Results demonstrated significant growth variations among scales. The laboratory bottle (5 L) achieved the highest cell density (54 × 10⁶ cells/mL) on day 11, attributed to optimal light distribution and stable temperature (30–33°C). Carboy cultures (10 L) showed moderate productivity (31 × 10⁶ cells/mL), while the intermediate scale (700–800 L) yielded the lowest density (6.8 × 10⁶ cells/mL) due to light limitation and suboptimal aeration. Water quality parameters remained stable across all systems, with pH 8.0, salinity 30–34 ppt, and uniform light intensity (500 lux). Statistical analysis confirmed significant differences in growth rates (p < 0.05), highlighting the inverse relationship between scale size and productivity. The study concludes that small-scale systems offer superior environmental control for C. vulgaris cultivation. For mass production, design improvements—such as enhanced aeration, closed photobioreactors, and artificial lighting—are recommended to replicate laboratory-scale efficiency. These findings provide actionable insights for optimizing microalgae culture systems in aquaculture feed production.References
Aeni, N., & Diamahesa, W. A. (2024). Evaluation of Common Carp (Cyprinus carpio) Cultivation Techniques at Balai Benih Ikan (BBI) Lingsar, West Nusa Tenggara. Journal of Fish Health, 4(1), 1-8.
Aprilliyanti, S., Soeprobowati, T. R., & Yulianto, B. (2016). Hubungan Kemelimpahan Chlorella sp. dengan Kualitas Lingkungan Perairan pada Skala Semi Masal di BBPBAP Jepara. Jurnal ilmu lingkungan, 14(2), 77-81.
APHA. (2017). Standard methods for the examination of water and wastewater (23rd ed.). American Public Health Association.
Aulia, D., & Diamahesa, W. A. (2024). Manajemen Kualitas Air Pada Pembesaran Kepiting Bakau (Scylla Sp.) Sistem Apartemen di Balai Besar Perikanan Budidaya Air Payau (BBPBAP) Jepara, Jawa Tengah. Ganec Swara, 18(2), 896-902.
Boroh, R., Litaay, M., Umar, M. R., & Ambeng, A. (2019). Pertumbuhan Chlorella sp. pada Beberapa Kombinasi Media Kultur. Jurnal Biologi Makassar, 4(2), 129-137.
Dayana, M. E., Singkam, A. R., & Jumiarni, D. (2022). Keanekaragaman Mikroalga sebagai Bioindikator di Perairan Sungai. Bioedusains: Jurnal Pendidikan Biologi dan Sains, 5(1), 77-84.
Maudina, F., & Diamahesa, W. A. (2023). Evaluation of Milkfish (Chanos chanos) Breeding Activities at the Balai Besar Perikanan Budidaya Air Payau (BBPBAP) jepara. Journal of Fish Health, 3(2), 52-59.
Hanifah, N., Heriyanto, Y., Anggrawati, H., & Fatikhah, N. (2021). Gambaran Pemahaman Tentang Sterilisasi Alat Kesehatan Gigi pada Mahasiswa Tingkat II Jurusan Keperawatan Gigi. Jurnal Kesehatan Siliwangi, 2(1), 362-368.
Istirokhatun, T., Aulia, M., & Sudarno (2017). Potensi Chlorella sp. untuk Menyisihkan COD dan Nitrat dalam. Jurnal Presipitasi: Media Komunikasi dan Pengembangan Teknik Lingkungan, 14(2), 88.
Mufidah, A., Agustono, S., & Nindarwi, D. D. (2017). Teknik Kultur Chlorella sp. Skala Laboratorium dan Intermediate di Balai Perikanan Budidaya Air Payau (BPBAP) Situbondo Jawa timur. Journal of Aquaculture and Fish Health, 7(2), 50-56.
Novianti, T., Zainuri, M., & Widowati, I. (2017). Studi Tentang Pertumbuhan Mikroalga Chlorella vulgaris yang Dikultivasi Berdasarkan Sumber Cahaya yang Berbeda. Jurnal Mangifera Edu, 1(2), 1-8.
Nisa, H., Diamahesa, W. A., & Lestari, D. P. (2024). Optimizing The Utilization of Arabica Coffe Grounds Extract on the Growth Perfomance of Tilapia (Oreochromis Niloticus). Jurnal Biologi Tropis, 24(4), 703-711.
Octhreeani, A. M., & Soedarsono, P. (2014). Pengaruh perbedaan jenis pupuk terhadap pertumbuhan Nannochloropsis sp. dilihat dari kepadatan sel dan klorofil α pada skala semi massal. Management of Aquatic Resources Journal (MAQUARES), 3(2), 102-108.
Prayogo, I., & Arifin, M. (2015). Teknik Kultur Pakan Alami Chlorella sp. dan Rotifera sp. Skala Massal dan Manajemen Pemberian Pakan Alami pada Larva Kerapu Cantang. Jurnal Ilmu Perikanan, 6(2), 127-134.
Rahman, N. C., (2015). Manajemen Pertumbuhan Kultur Mikroalga Laut Nannochloropsis oculata Skala Laboratorium, dan Intermediate di Balai Perikanan Budidaya Air Payau (BPBAP) SITUBONDO. Jurnal Perikanan dan Ilmu Kelautan, 2(1), 9-10.
Rihi, A. P. (2019). Pengaruh Pemberian Pakan Alami dan Buatan terhadap Pertumbuhan dan Kelangsungan Hidup Benih Ikan Lele Dumbo (Clarias gariepinus Burchell.) di Balai Benih Sentral Noekele Kabupaten Kupang. Bio-Edu: Jurnal Pendidikan Biologi, 4(2), 59-68.
Risna, Y. K., Sri-Harimurti, S. H., Wihandoyo, W., & Widodo, W. (2022). Kurva Pertumbuhan Isolat Bakteri Asam Laktat dari Saluran Pencernaan Itik Lokal Asal Aceh. Jurnal peternakan indonesia, 24(1), 1-7.
Rosyadi, R., Agusnimar, A., & Hadi, K. (2023). Pemanfaatan Hasil Fermentasi Lindi dengan Konsentrasi yang Berbeda terhadap Pertumbuhan dan Kepadatan Sel Chlorella sp. Media Akuakultur, 18(2), 47-53.
Tsany, M. R. N. (2016). Kultur Fitoplankton Tetraselmis sp. Skala Laboratorium sebagai Pakan Rotifer (Brachionus sp.) di Sriracha Fisheries Research Station, Chonburi, Thailand.
YUSRIN, Y., & DIAMAHESA, W. A. (2024). Teknik pembenihan ikan lele (Clarias gariepinus) di Instalasi Balai Benih Ikan Lingsar. GANEC SWARA, 18(2), 917-924.
Zulfiana, Z. (2019). Penentuan Kepadatan Chlorella sp. Menggunakan Software Color Analysis (SOCA) dI HATCHERY (Doctoral dissertation, Universitas Hasanuddin).